ABSTRACT
The aim of this study was to compare superoxide dismutase [SOD] activity in Fasciola hepatica and fasciola gigantica parasites. F. gigantica and F. hepatica helminths were collected from abattoir and cultured in buffer media for 4 h at 37 °C. Excretory-Secretory [ES] products were collected, centrifuged and stored at 20°C. E-S protein concentration was measured by Bradford method and SOD activity was detected using RANSOD kit [Randox Lab. Crumlin, UK]. Statistical t-test was conducted for analysis of results. Protein concentration for F. hepatica and F. gigantica were obtained 7.293 ug/ml and 19.65 ug/ml respectively and SOD activity as 0.721 U/ml and 1.189 U/ml, in that order. ES protein concentration of two species was significantly different [P<0.05], however the difference of SOD activity of two species was not significant. Two species of Fasciola have comparable SOD biochemical defense enzyme and can help us explain the parasite survival in host tissue
ABSTRACT
The purpose of this comparative study was to detect superoxide dismutase [SOD] activities in Fasciola hepatica, F. gigantica parasites, infected and healthy liver tissues in order to determine of species effects and liver infection on SODs activity level. Fasciola spp. parasites and sheep liver tissues [healthy and infected liver tissues], 10 samples for each, were collected, homogenized and investigated for protein measurement, protein detection and SOD enzyme activity assay. Protein concentration was measured by Bradford method and SODs band protein was detected on SDS-PAGE. SODs activity was determined by iodonitrotetrazolium chloride, INT, and xanthine substrates. Independent samples t-test was conducted for analysis of SODs activities difference. Protein concentration means were detected for F. hepatica 1.3 mg/ ml, F. gigantica 2.9 mg/ml, healthy liver tissue 5.5 mg/ml and infected liver tissue 1.6 mg/ml [with similar weight sample mass]. Specific enzyme activities in the samples were obtained 0.58, 0.57, 0.51, 1.43 U/mg for F. hepatica, F. gigantica, healthy liver and infected liver respectively. Gel electrophoresis of Fasciola spp. and sheep liver tissue extracts revealed a band protein with MW of 60 kDa. The statistical analysis revealed significant difference between SOD activities of Fasciola species and also between SOD activity of liver tissues [P<.05]. Fasciola species and liver infection are effective causes on SOD enzyme activity level
Subject(s)
Animals , Fasciola hepatica , Superoxide Dismutase , Liver Diseases, Parasitic , Liver/parasitologyABSTRACT
Purpose: to compare the intraocular lens [IOL] power, keratometric values, and axial length of both eyes
Methods: in a case series, 100 patients were selected by simple randomized sampling. Keratometry was performed by Goldman keratometer, axial length was measured with contact method, and PC IOL power was calculated with A constant 118.5 by SRK II formula in both eyes of all patients. Inclusion criteria were axial length of 22-26 mm, an isometropia 50.50 diopter, age 315 years, and good patient cooperation. The values of both eyes were compared and statistically analyzed
Results: keratometry, axial length, and intraocular lens power of fellow eyes had no clinically significant differences. Axial length of eyes in men was significantly longer than women. Mean keratometric values of women's eyes was significantly more than men. There was no significant difference in intraocular lens power between men and women. The IOL power difference was 51 diopter in 89% of cases and 52 diopters in 99%. The IOL power difference was 0.61 +/- 0.65 diopter in women and 0.45 +/- 0.53 diopter in men which was not statistically significant
Conclusion: there are usually acceptable results of IOL power calculation by using fellow eye criteria. This can be used in medium sized eyes with less than 0.5 diopter an isometropia. Sometimes there is a surprise result, which may be related to precision of used formula or experience of examiner